Application

Milk samples from 10 mothers were probed for bound milk IgA by western blot using alkaline-phosphatase goat anti-human IgA antibody.Elisa Assays were performed using alkaline phosphatase conjugated gaot anti-human IgA chain specific antibody (1:1000) to detect IgA immunoglobulins in serum and seminal plasma samples from infertile men.

Human milk samples were analyzed for the presence of IgA antibodies specific to certain antigens of H. pylori by western blot using alkaline-phosphate-conjugated goat anti-human IgA antibody.

Anti-Human IgA (α-chain specific) Alkaline Phosphatase antibody produced in goat has been used in quantitative sandwich enzyme-linked immunosorbent assay (ELISA) and immunoassays.

The product may also be used for dot blot (1:30,000) and immunohistochemistry (at 1:100 dilutions, using formalin-fixed, paraffin-embedded sections).

Biochem/physiol Actions

Immunoglobulin A (IgA) antibody isasecretoryantibody presentabundantly in mucous linings of gastrointestinal, respiratory and genitourinary tracts, tears and saliva. Thus, secretory IgA contributes to the humoraldefensemechanism against the pathogens on mucosalsurfaces.

IgA is a secretory glycoprotein antibody that regulates the physiological balance between commensal intestinal bacteria and the defenses of the host immune system. It is the highest generated antibody isotype. IgAs are mainly secreted in the intestinal mucosa and prevent the commensal microorganisms from invading the intestines

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Immunoglobulin A (IgA) is the most abundant antibody isotype produced inhumans. IgAs are heterogeneous and exist as twoIgA subtypes. It has the ability to form multimers by interacting with the joining (J) chain.It is present in large quantities in blood. IgA antibodies protect mucosal surfaces against invading pathogens.

Specificity of the Alkaline Phosphatase Conjugated Anti-Human IgA is determined by Enzyme Linked Immunosorbent Assay (ELISA). Identity and purity of the antibody is established by immunoelectrophoresis (IEP) prior to conjugation. Electrophoresis of the antibody preparation followed by diffusion versus anti-goat IgG and anti-goat whole serum results in single arcs of precipitation.

Immunogen

Purified human IgA

Physical form

Solution in 0.05 M Tris, pH 8.0, containing 1% bovine serum albumin, 1 mM MgCl₂ and 15 mM sodium azide

Specificity

The conjugate is specific for human IgG. No cross-reaction with Mouse and Rat IgG.

Storage and Stability

Store at 0-5°C. Do Not Freeze.